ampliscribe t7 high yield transcription kit (Lucigen Corp)

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Lucigen Corp ampliscribe t7 high yield transcription kit
Ampliscribe T7 High Yield Transcription Kit, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ampliscribe+t7+high+yield+transcription+kit/ampliscribe+flash+kit+t7+transcription/pmc13139853-180-10-16
Average 86 stars, based on 1 article reviews

ampliscribe t7 high yield transcription kit - by Bioz Stars, 2026-10

86/100 stars

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In Vitro:

Article Title: Seasonal and comparative evidence of adaptive gene expression in mammalian brain size plasticity
Article Snippet: .. The AmpliScribe T7 High Yield Transcription kit (Lucigen) was used for RNA in vitro transcription with a 2 hr incubation at 37°C using gblock sequences available in supplements. .. BCL2L1 sequences were designed with a T7 promoter inserted at the beginning to facilitate transcription (Integrated DNA Technologies).

Article Title: The clade-specific target recognition mechanisms of plant RISCs
Article Snippet: .. The mRNA coding for the catalytic mutant AGOs used in the equilibrium binding assay was transcribed in vitro using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), employing the corresponding pBYL-3 × FLAG-SUMO-catalytic mutant AGO plasmids linearized with Not I as templates. ..

Article Title: An anti-aggregation region of the SGS3 N-terminal IDR is essential for secondary siRNA biogenesis
Article Snippet: DNA fragment was directly introduced to E. coli competent cells. pEarleyGate-3xHA_EGFP, pEarleyGate-3xHA_SGS3_EGFP, pEarleyGate-3xHA_ΔPrLD_SGS3_EGFPΔPrLD, pEarleyGate-3xHA_Δ160_SGS3_EGFP, pEarleyGate-3xHA_Δ200_SGS3_EGFP, pEarleyGate-3xHA_Δ161-200_SGS3_EGFP, pEarleyGate-3xHA_ΔNCP_SGS3_EGFP, pEarleyGate-3xHA_Δ191-200_SGS3_EGFP pENTA-3xHA_EGFP, pENTA-3xHA_SGS3_EGFP, pENTA-3xHA_ΔPrLD_SGS3_EGFP, pENTA-3xHA_Δ160_SGS3_EGFP, pENTA-3xHA_Δ200_SGS3_EGFP, pENTA-3xHA_Δ 161-200_SGS3_EGFP, pENTA-3xHA_ΔNCP_SGS3_EGFP, and pENTA-3xHA_Δ191-200_SGS3_EGFP plasmids were inserted into pEarleyGate 100 vector by using LR Clonase (Invitrogen) reaction-mediated recombination system. .. mRNAs were transcribed in vitro from NotI- (for plasmids with the prefix ‘‘pBYL-’’) or XhoI-(for plasmids with the prefix ‘‘pUC57-’’) digested plasmids or PCR products amplified from pENTA-plasmids by using Oligo11 and Oligo12 primer set using the AmpliScribe T7 High Yield Transcription Kit (Lucigen) or T7-Scribe TM Standard RNA IVT Kit (Cell Script), followed by capping with ScriptCap m7G Capping System (Cell Script). ..

Article Title: Cleavage region organizes the structural architecture of the B2 SINE ribozyme
Article Snippet: The gBlock was subjected to polymerase chain reaction using Ex Taq high fidelity DNA Polymerase (Takara Bio USA, # RR001A) and the following primers 5’-TAATACGACTCACT ATAG-3 and as a reverse primer for the B2 RNA PCR template, the following sequence 5’-TTTTTTTTTA AAGATTTTTTATTTATTATATGTAAGTACA-3’. .. In vitro transcription reactions were run using the AmpliScribe T7 High Yield Transcription Kit (Lucigen AS3107) for 4 hours at 42°C, followed by a 15 min incubation with DNase I at 37°C. .. Reaction products were diluted to 1 mL with size exclusion buffer (8 mM MOPS pH 6.5, 100 mM KCl, 0.1 mM EDTA) and purified in an AKTA Pure 25 system on a Superdex 200 Increase 10/300 GL column (GE Healthcare).

Article Title: Cleavage region organizes the structural architecture of the SINE-derived B2 repressive ribozyme
Article Snippet: The gBlock was subjected to polymerase chain reaction using Ex Taq high-fidelity DNA Polymerase (Takara Bio USA, # RR001A) and the following primers 5’-TAATACGACTCACTATAG- 3 and, as a reverse primer for the B2 RNA PCR template, the following sequence 5’-TTTTTTTTTA AAGATTTTTTATTTATTATATGTAAGTACA-3’. .. In vitro transcription reactions were run using the AmpliScribe T7 High Yield Transcription Kit (Lucigen AS3107) for 4 h at 42 °C, followed by a 15-min incubation with DNase I at 37 °C. .. Reaction products were diluted to 1 mL with size exclusion buffer (8 mM MOPS pH 6.5, 100 mM KCl, 0.1 mM EDTA) and purified in an AKTA Pure 25 system on a Superdex 200 Increase 10/300 GL column (GE Healthcare).

Article Title: Cleavage region organizes the structural architecture of the SINE-derived B2 repressive ribozyme.
Article Snippet: The gBlock was subjected to polymerase chain reaction using Ex Taq high-fidelity DNA Polymerase (Takara Bio USA, # RR001A) and the following primers 5’-TAATACGACTCACTATAG- 3 and, as a reverse primer for the B2 RNA PCR template, the following sequence 5’-TTTTTTTTTA AAGATTTTTTATTTATTATATGTAAGTACA-3'. .. In vitro transcription reactions were run using the AmpliScribe T7 High Yield Transcription Kit (Lucigen AS3107) for 4 hours at 42°C, followed by a 15- minute incubation with DNase I at 37°C. .. Reaction products were diluted to 1 mL with size exclusion buffer (8 mM MOPS pH 6.5, 100 mM KCl, 0.1 mM EDTA) and purified in an AKTA Pure 25 system on a Superdex 200 Increase 10/300 GL column (GE Healthcare).

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Article Title: Functional interaction surfaces of the bacterial transcription terminator Rho are required for the interactions with the elongation factor NusG.
Article Snippet: AmpliScribe T7 High Yield Transcription Kit from Lucigen.

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